Clinical Chemistry
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Clinical Chemistry 29: 290-296, 1983;
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Clinical Chemistry, Vol 29, 290-296, Copyright © 1983 by American Association for Clinical Chemistry

Characterization of bacteria by mixed-dye fluorometry

DC Shelly, IM Warner and JM Quarles

We describe an indirect detection method for bacterial identification and differentiation, based on selective adsorption of several fluorescent dyes. The lipid, protein, and nucleic acid components of fixed whole cells are stained with two mixtures, each containing two fluorochromes. The unadsorbed dyes were measured simultaneously with a video fluorometer [Clin Chem 22: 1483, 1976]. A dye-absorption matrix of the response can be generated, and we did so for each of nine bacteria. These responses were compared to a control or "complete" response matrix, and the response ratios of each bacterial species for each of the four dyes were calculated and plotted to obtain a characteristic pattern. From the response-ratio plots, plus simple pattern-recognition techniques, we could differentiate among all the bacteria. This rapid, sensitive technique is potentially applicable to a wide variety of bacteria.




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Copyright © 1983 by the American Association for Clinical Chemistry.