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Clinical Chemistry 29: 302-304, 1983;
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Clinical Chemistry, Vol 29, 302-304, Copyright © 1983 by American Association for Clinical Chemistry

Centrifugal analyzer used for enzyme immunoassay of progesterone and choriomammotropin

B Terouanne, J Marchand, C Calzolari, J Monnier, JC Nicolas, B Pau and B Descomps

Recently we developed an enzyme immunoassay involving the use of steroid delta-isomerase (EC 5.3.3.1) as enzyme label and exclusion- affinity chromatography for rapid separation of free antigen-enzyme conjugate that bound to antibodies (J. Immunol. Methods 35: 267-284, 1980). Here we describe an automated version of this procedure, for immunoassay of progesterone and choriomammotropin (human placental lactogen) in serum with the use of a centrifugal analyzer. After incubation, suitable dilutions of sera or extract plus antiserum, conjugate, and double antibody were filtered on an estradiol affinity gel-filtration column; the enzyme activity of the filtrates was determined with the centrifugal analyzer. Results correlate well with those obtained by radioimmunoassay: r (progesterone) = 0.980, r (choriomammotropin) = 0.940. The within-run and between-run precision, specificity, sensitivity, accuracy, and speed of this system make it a useful tool for immunoassay.




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Copyright © 1983 by the American Association for Clinical Chemistry.