Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 29: 305-309, 1983;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Orsulak, P. J.
Right arrow Articles by Schildkraut, J. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Orsulak, P. J.
Right arrow Articles by Schildkraut, J. J.

Clinical Chemistry, Vol 29, 305-309, Copyright © 1983 by American Association for Clinical Chemistry

Determination of urinary normetanephrine and metanephrine by radial- compression liquid chromatography and electrochemical detection

PJ Orsulak, P Kizuka, E Grab and JJ Schildkraut

A procedure has been developed for determining the O-methylated catecholamine metabolites, normetanephrine and metanephrine, in urine by use of radial-compression liquid chromatography followed by electrochemical detection. Normetanephrine and metanephrine are isolated from hydrolyzed urine by ion-exchange on small, commercially available, disposable columns and preconcentrated by solvent extraction. They are then separated by reversed-phase ion-pair chromatography, with use of a radial compression cartridge and radial compression module, and quantified with 3-methoxy-4-hydroxybenzylamine as internal standard. Normetanephrine, metanephrine, and the internal standard are separated from interfering peaks in about 15 min. The method is applicable to the relatively low amounts of normetanephrine (100-600 micrograms/24 h) and metanephrine (50-400 micrograms/24 h) found in normal subjects and patients with depressive disorders or hypertension. Within-day CVs ranged from 1.1 to 2.2% for normetanephrine and 1.2 to 6.9% for metanephrine; the corresponding between-day CVs were 4.9 and 5.7% over these ranges.


The following articles in journals at HighWire Press have cited this article:


Home page
 Arch Gen PsychiatryHome page
A. F. Schatzberg, J. A. Samson, K. L. Bloomingdale, P. J. Orsulak, B. Gerson, P. P. Kizuka, J. O. Cole, and J. J. Schildkraut
Toward a Biochemical Classification of Depressive Disorders: X. Urinary Catecholamines, Their Metabolites, and D-Type Scores in Subgroups of Depressive Disorders
Arch Gen Psychiatry, March 1, 1989; 46(3): 260 - 268.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1983 by the American Association for Clinical Chemistry.