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Clinical Chemistry 29: 343-345, 1983;
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Clinical Chemistry, Vol 29, 343-345, Copyright © 1983 by American Association for Clinical Chemistry

Assessment of the benzethonium chloride method for routine determination of protein in cerebrospinal fluid and urine

E Flachaire, O Damour, J Bienvenu, T Aouiti and R Later

We have tested the characteristics of the method of Iwata and Nishikaze (Clin Chem 25: 1317, 1979). The linearity, sensitivity, and precision are satisfactory and the reactivity of benzethonium chloride with various proteins (albumin, immunoglobulins) is the same. The method has been compared with Meulemans's technique (Clin Chim Acta 5: 757, 1960), routinely used in our laboratories, by analysis of 82 samples of cerebrospinal fluid (CSF) and 119 samples of urine. Our results for cerebrospinal fluid agree well with those of Iwata and Nishikaze (r = 0.976; y = 0.992x - 0.013), but we find their method unsuitable for urinary protein determination, probably because of interfering compounds in urine.


The following articles in journals at HighWire Press have cited this article:


Home page
Clin. Chem.Home page
F. M. Yilmaz, N. Celebi, and D. Yucel
Automated Turbidimetric Benzalkonium Chloride Method for Measurement of Protein in Urine and Cerebrospinal Fluid
Clin. Chem., August 1, 2004; 50(8): 1450 - 1452.
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