Clinical Chemistry
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Clinical Chemistry 29: 415-426, 1983;
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Clinical Chemistry, Vol 29, 415-426, Copyright © 1983 by American Association for Clinical Chemistry

Some considerations of methodology and standardization of apolipoprotein A-I immunoassays

KK Steinberg, GR Cooper, SR Graiser and M Rosseneu

Apolipoprotein A-I (apo A-I) is the major protein of high-density lipoprotein. Compelling evidence suggests that measurement of concentrations of apo A-I in serum may be useful in predicting and assessing ischemic heart disease. The following review of methods of isolation, characterization, and assay of apo A-I has been developed to assess the possibility of standardizing apo A-I immunoassays. We consider the properties of apo A-I that have an effect on its quantification, such as self-association, polymorphism, and stability characteristics. We attempt to review critically the various methods presented, but more information about the physicochemical properties of the protein is required before definitive recommendations can be made.


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Copyright © 1983 by the American Association for Clinical Chemistry.