Clinical Chemistry
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Clinical Chemistry 29: 491-494, 1983;
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Clinical Chemistry, Vol 29, 491-494, Copyright © 1983 by American Association for Clinical Chemistry

Improved measurement of acetylsalicylic acid esterase in serum

S Sorensen

A fixed-time incubation method for measuring acetylsalicylic acid esterase was improved by using a higher concentration of the substrate, acetylsalicylic acid, and an activator, Ca2+. The enzymatic activity in serum was 25-fold that measured by earlier methods. Inhibition studies showed a pattern similar to that reported for cholinesterase. Use of cholinesterase inhibitor in sample tubes for determination of acetylsalicylic acid and salicylic acid is recommended.




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Copyright © 1983 by the American Association for Clinical Chemistry.