Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 29: 656-663, 1983;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Demacker, P. N.
Right arrow Articles by van't Laar, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Demacker, P. N.
Right arrow Articles by van't Laar, A.

Clinical Chemistry, Vol 29, 656-663, Copyright © 1983 by American Association for Clinical Chemistry

Ultracentrifugation in swinging-bucket and fixed-angle rotors evaluated for isolation and determination of high-density lipoprotein subfractions HDL2 and HDL3

PN Demacker, DF van Sommeren-Zondag, AF Stalenhoef, PM Stuyt and A van't Laar

We evaluated the density-gradient ultracentrifugation method in a swinging-bucket rotor (Anal Biochem 111, 149-157, 1981) in a slightly modified version for isolation and determination of high-density lipoprotein (HDL) subfractions. We prestained the serum with Coomassie Brilliant Blue R, which did not change the hydrated densities of the lipoproteins, and after only 2.2 X 10(8) gav . min obtained an equilibrium distribution of the lipoproteins along the gradient. The density distribution of the HDL of 120 sera obtained from apparently healthy persons and from patients with different types of hyperlipoproteinemia was bimodal. The HDL2 could be isolated in the density range 1.072-1.098 kg/L and the HDL3 at 1.100-1.176 kg/L, the latter fraction being more heterogeneous. At a solvent density of 1.100 we obtained similar results for HDL2-and HDL3-cholesterol by ultracentrifugation in two different fixed-angle rotors with tube angles of 15 degrees or 35 degrees. Independent of the rotor and the ultracentrifugation technique, subfractionation at d = 1.100 resulted in more distinct stained entities than ultracentrifugation at d = 1.125. In the swinging-bucket rotor procedure, interference by sinking pre-beta-lipoproteins was minimized because, having hydrated densities between 1.058 and 1.075, they could be removed without aspirating the HDL2. The method is both accurate and precise. For HDL2- and HDL3- cholesterol determined in a thawed frozen serum pool, CVs were 8.8 and 6.3%, respectively (n = 18).


The following articles in journals at HighWire Press have cited this article:


Home page
 Ann Rheum DisHome page
C Popa, L J H van Tits, P Barrera, H L M Lemmers, F H J van den Hoogen, P L C M van Riel, T R D J Radstake, M G Netea, M Roest, and A F H Stalenhoef
Anti-inflammatory therapy with tumour necrosis factor alpha inhibitors improves high-density lipoprotein cholesterol antioxidative capacity in rheumatoid arthritis patients
Ann Rheum Dis, June 1, 2009; 68(6): 868 - 872.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
I. J. Martins, E. Hone, C. Chi, U. Seydel, R. N. Martins, and T. G. Redgrave
Relative roles of LDLr and LRP in the metabolism of chylomicron remnants in genetically manipulated mice
J. Lipid Res., February 1, 2000; 41(2): 205 - 213.
[Abstract] [Full Text]

Home page
 Am. J. Clin. Nutr.Home page
L. J van Tits, P. N Demacker, J. de Graaf, H. L Hak-Lemmers, and A. F Stalenhoef
{alpha}-Tocopherol supplementation decreases production of superoxide and cytokines by leukocytes ex vivo in both normolipidemic and hypertriglyceridemic individuals1
Am. J. Clinical Nutrition, February 1, 2000; 71(2): 458 - 464.
[Abstract] [Full Text] [PDF]

Home page
 Innate ImmunityHome page
M. G. Netea, P. N.M. Demacker, T. Verver-Jansen, L. Jacobs, B. Jan Kullberg, A. F.H. Stalenhoef, and J. W.M. Van der Meer
The dense low-density lipoprotein (LDL) subfractions are more potent than buoyant LDL for binding and neutralization of lipopolysaccharide
Innate Immunity, December 1, 1997; 4(6): 409 - 413.
[Abstract] [PDF]

Home page
 Clin. Chem.Home page
P. N. M. Demacker, M. Hessels, H. Toenhake-Dijkstra, and H. Baadenhuijsen
Precipitation methods for high-density lipoprotein cholesterol measurement compared, and final evaluation under routine operating conditions of a method with a low sample-to-reagent ratio
Clin. Chem., April 1, 1997; 43(4): 663 - 668.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
J. A. Kuivenhoven, A. F.H. Stalenhoef, J. S. Hill, P. N.M. Demacker, A. Errami, J. J.P. Kastelein, and P. H. Pritchard
Two Novel Molecular Defects in the LCAT Gene Are Associated With Fish Eye Disease
Arterioscler Thromb Vasc Biol, February 1, 1996; 16(2): 294 - 303.
[Abstract] [Full Text]

Home page
 Arch DermatolHome page
J. A. Gomez, M. J. Gonzalez, J. M. de Moragas, J. Serrat, F. Gonzalez-Sastre, and M. Perez
Apolipoprotein E Phenotypes, Lipoprotein Composition, and Xanthelasmas
Arch Dermatol, August 1, 1988; 124(8): 1230 - 1234.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1983 by the American Association for Clinical Chemistry.