Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 29: 667-671, 1983;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schroeder, H. R.
Right arrow Articles by Halter, C. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schroeder, H. R.
Right arrow Articles by Halter, C. M.

Clinical Chemistry, Vol 29, 667-671, Copyright © 1983 by American Association for Clinical Chemistry

Specificity of human beta-choriogonadotropin assays for the hormone and for an immunoreactive fragment present in urine during normal pregnancy

HR Schroeder and CM Halter

We evaluated four high-purity commercial preparations of human choriogonadotropin (hCG; Boehringer-Mannheim, Calbiochem, Cambridge, and Radioassay Systems) by two radioimmunoassays and one radioreceptor procedure. Their specific activities were less than half that of the first International Reference Preparation of hCG for immunoassay. In addition, a fragment that was immunoreactive in radioimmunoassays for hCG with antisera to the beta-subunit conformational determinant was isolated from crude hCG (Roussel Corp.). The fragment adsorbed to concanavalin A and exhibited a relative molecular mass of 12 000 by gel filtration. In a study with 94 urines from women three to 24 weeks pregnant, the fragment contributed more than 70% of the immunoreactivity detected by the above radioimmunoassays for hCG in 70% of the samples. The fragment was present in urine throughout pregnancy, but was not detected in the serum of any of seven pregnant women tested.


The following articles in journals at HighWire Press have cited this article:


Home page
 J. Clin. Endocrinol. Metab.Home page
M. Nemansky, E. Moy, C. D. Lyons, I. Yu, and D. L. Blithe
Human Endometrial Stromal Cells Generate Uncombined {alpha}-Subunit from Human Chorionic Gonadotropin, Which Can Synergize with Progesterone to Induce Decidualization
J. Clin. Endocrinol. Metab., February 1, 1998; 83(2): 575 - 581.
[Abstract] [Full Text]

Home page
 Clin. Chem.Home page
U.-H. Stenman, L. Unkila-Kallio, J. Korhonen, and H. Alfthan
Immunoprocedures for detecting human chorionic gonadotropin: clinical aspects and doping control
Clin. Chem., July 1, 1997; 43(7): 1293 - 1298.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Feng, M. M. Matzuk, K. Mountjoy, E. Bedows, R. W. Ruddon, and I. Boime
The Asparagine-linked Oligosaccharides of the Human Chorionic Gonadotropin [IMAGE] Subunit Facilitate Correct Disulfide Bond Pairing
J. Biol. Chem., May 19, 1995; 270(20): 11851 - 11859.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1983 by the American Association for Clinical Chemistry.