Clinical Chemistry
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Clinical Chemistry 29: 677-680, 1983;
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Clinical Chemistry, Vol 29, 677-680, Copyright © 1983 by American Association for Clinical Chemistry

Immunoadsorption to improve gas chromatography/high-resolution mass spectrometry of estradiol-17 beta in plasma

SJ Gaskell and BG Brownsey

We describe a new, highly selective procedure for the determination of estradiol-17 beta in plasma. Samples are extracted with a micro- cellulose-coupled antiserum to estradiol-17 beta. Conversion of the extracted steroid to the bis(trimethylsilyl) ether is followed by gas chromatography/high-resolution mass spectrometry with selected ion monitoring. Precise quantification is achieved through the use of [2H3]estradiol-17 beta as internal standard.


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 Clin. Chem.Home page
Z. Cao, T. A. Swift, C. A. West, T. G. Rosano, and R. Rej
Immunoassay of Estradiol: Unanticipated Suppression by Unconjugated Estriol
Clin. Chem., January 1, 2004; 50(1): 160 - 165.
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 Clin. Chem.Home page
H. Wu, C. Ramsay, P. Ozaeta, L. Liu, and H. Aboleneen
Serum Estradiol Quantified by Isotope Dilution-Gas Chromatography/Mass Spectrometry
Clin. Chem., February 1, 2002; 48(2): 364 - 366.
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Copyright © 1983 by the American Association for Clinical Chemistry.