Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 29: 806-809, 1983;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Blaton, V.
Right arrow Articles by Declercq, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Blaton, V.
Right arrow Articles by Declercq, B.

Clinical Chemistry, Vol 29, 806-809, Copyright © 1983 by American Association for Clinical Chemistry

Enzymic assay for phosphatidylcholine and sphingomyelin in serum

V Blaton, M De Buyzere, J Spincemaille and B Declercq

We describe a method for the direct enzymatic determination of phosphatidylcholine and sphingomyelin in serum. Phospholipase C (EC 3.1.4.3) and sphingomyelinase (EC 3.1.4.12) are used to hydrolyze phosphatidylcholine and sphingomyelin, respectively, with high specificity; alkaline phosphatase (EC 3.1.3.1) is used to cleave inorganic phosphorus. The choline group, after oxidation with choline oxidase (EC 1.1.3.17), is detected with 4-aminoantipyrine. Alternatively, phosphorus is assayed with metavanadate. The excellent agreement between results of this modification of a procedure described by Artiss et al. (Microchem. J. 26: 1017, 1980) for amniotic fluid and of the conventional thin-layer chromatographic method makes this an attractive method for determination of both phospholipid subclasses in serum.


The following articles in journals at HighWire Press have cited this article:


Home page
 J. Lipid Res.Home page
M. N. Nanjee, C. J. Cooke, W. L. Olszewski, and N. E. Miller
Lipid and apolipoprotein concentrations in prenodal leg lymph of fasted humans: associations with plasma concentrations in normal subjects, lipoprotein lipase deficiency, and LCAT deficiency
J. Lipid Res., August 1, 2000; 41(8): 1317 - 1327.
[Abstract] [Full Text]

Home page
 J CARDIOVASC PHARMACOL THERHome page
S. Lepage, F. Nigon, D. Bonnefont-Rousselot, U. Assogba, S. Goulinet, L. Chancharme, J. Delattre, E. Bruckert, and M. J. Chapman
Oxidizability of Atherogenic Low-Density Lipoprotein Subspecies in Severe Familial Hypercholesterolemia: Impact of Long-Term Low-Density Lipoprotein Apheresis
Journal of Cardiovascular Pharmacology and Therapeutics, January 1, 2000; 5(2): 87 - 103.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1983 by the American Association for Clinical Chemistry.