Clinical Chemistry
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Clinical Chemistry 29: 836-839, 1983;
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Clinical Chemistry, Vol 29, 836-839, Copyright © 1983 by American Association for Clinical Chemistry

Modified technique for periodic acid-Schiff staining of glycoproteins on agarose-film electrophoretograms

SM Trivedi, CG Frondoza and RL Humphrey

We have modified the periodic acid-Schiff staining technique for glycoproteins for use with thin agarose-film electrophoresis membranes. With this procedure, carbohydrate-rich proteins can be detected with negligible background staining and insignificant staining of nonglycoproteins such as albumin and nonglycosylated Bence Jones proteins (free light chains). On the other hand, carbohydrate-rich M components of immunoglobulins M and A in serum and in cerebrospinal fluid from patients with plasma cell dyscrasia are readily detected. The technique is two- to threefold more sensitive than Ponceau S. Glycoproteins in serum and body fluids can be determined as a routine analytical procedure.




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Copyright © 1983 by the American Association for Clinical Chemistry.