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Clinical Chemistry 29: 1024-1027, 1983;
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Clinical Chemistry, Vol 29, 1024-1027, Copyright © 1983 by American Association for Clinical Chemistry

125I-labeled radioimmunoassay kits for progesterone evaluated for use in an vitro fertilization program

LF Blight and GH White

We have evaluated two commercially available 125I radioimmunoassay kits (Diagnostic Products Corp., DPC; and Radioassay Systems Laboratories, RSL) for measurement of serum or plasma progesterone, to determine their suitability for use in in vitro fertilization programs. Both kits were suitably rapid for program requirements. Results by both were linear with concentration up to 60 nmol/L, and both had acceptable lower detection limits of 0.3 nmol/L. Kit-determined progesterone concentrations (y) for 100 patients' samples correlated well with results by our existing 3H radioimmunoassay method (y = 1.11x + 0.2, r = 0.965 for the DPC kit; y = 1.01x + 1.4, r = 0.974 for the RSL kit). Mean analytical recovery for the RSL kit was 116%, that for the DPC kit, 202%. Within-batch precision, expressed as the mean CV for three concentrations of progesterone, was 6.5% for the RSL kit, and 16.4% for the DPC kit; between-day CV was 8.1% for the RSL kit, 17.7% for the DPC kit. We conclude that the RSL kit provides a rapid, precise, and accurate assay for serum progesterone, suitable for use in a fertilization program, but do not recommend the DPC kit for either this purpose or the more general purpose of tracking menstrual cycles.




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Copyright © 1983 by the American Association for Clinical Chemistry.