Clinical Chemistry
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Clinical Chemistry 29: 1038-1041, 1983;
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Clinical Chemistry, Vol 29, 1038-1041, Copyright © 1983 by American Association for Clinical Chemistry

Reflectance digital matrix photometry

WE Neeley and A Zettner

We have designed and constructed a digital matrix photometer for quantitative measurement of reflected light of small chromophoric areas or colored spots. The areas are divided conceptually into small subunits in which the reflected light is measured. This is done through stepwise scanning with a photodiode array. The sum of the individual reflectance measurements is related to the total amount of an analyte in the spot. We have found that this technique makes possible accurate and precise quantitation of light-absorbing substances in small areas of different sizes, shapes, and internal irregularity measuring as little as 1 mm across.




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Copyright © 1983 by the American Association for Clinical Chemistry.