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Clinical Chemistry 29: 1075-1080, 1983;
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Clinical Chemistry, Vol 29, 1075-1080, Copyright © 1983 by American Association for Clinical Chemistry

Reagent for the enzymatic determination of serum total cholesterol with improved lipolytic efficiency

J Siedel, EO Hagele, J Ziegenhorn and AW Wahlefeld

We describe a sensitive method for quantifying the extent of cholesterol ester cleavage during enzymatic assay of total cholesterol in serum. Lipids are extracted from the assay mixture with chloroform/methanol (1/1 by vol), concentrated, then quantified by "high-performance" thin-layer chromatography. Although with conventional enzymatic reagents for determination of serum total cholesterol the hydrolysis of the cholesterol esters may be incomplete, a new enzymatic cholesterol reagent (Monotest Cholesterol, High Performance, Boehringer Mannheim) gives virtually complete cholesterol ester cleavage (i.e., greater than or equal to 99.5%). Use of this reagent with its improved lipolytic efficiency yields results for serum total cholesterol that are identical to those measured with a candidate reference procedure involving alkaline cholesterol ester saponification.


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[Abstract] [Full Text] [PDF]

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 BMJHome page
P. Primatesta and N. R Poulter
Lipid concentrations and the use of lipid lowering drugs: evidence from a national cross sectional survey
BMJ, November 25, 2000; 321(7272): 1322 - 1325.
[Abstract] [Full Text]

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 Am. J. Clin. Nutr.Home page
T. J Roseboom, J. H. van der Meulen, C. Osmond, D. J. Barker, A. C. Ravelli, and O. P Bleker
Plasma lipid profiles in adults after prenatal exposure to the Dutch famine
Am. J. Clinical Nutrition, November 1, 2000; 72(5): 1101 - 1106.
[Abstract] [Full Text] [PDF]

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 Am J EpidemiolHome page
R. Din-Dzietham, D. Liao, A. Diez-Roux, F. J. Nieto, C. Paton, G. Howard, A. Brown, M. Carnethon, and H. A. Tyroler
Association of Educational Achievement with Pulsatile Arterial Diameter Change of the Common Crotid Artery The Atherosclerosis Risk in Communities (ARIC) Study, 1987-1992
Am. J. Epidemiol., October 1, 2000; 152(7): 617 - 627.
[Abstract] [Full Text] [PDF]

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 ANN INTERN MEDHome page
I. Saito, A. R. Folsom, F. L. Brancati, B. B. Duncan, L. E. Chambless, and P. G. McGovern
Nontraditional Risk Factors for Coronary Heart Disease Incidence among Persons with Diabetes: The Atherosclerosis Risk in Communities (ARIC) Study
Ann Intern Med, July 18, 2000; 133(2): 81 - 91.
[Abstract] [Full Text] [PDF]

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 CirculationHome page
Q. Xu, G. Schett, H. Perschinka, M. Mayr, G. Egger, F. Oberhollenzer, J. Willeit, S. Kiechl, and G. Wick
Serum Soluble Heat Shock Protein 60 Is Elevated in Subjects With Atherosclerosis in a General Population
Circulation, July 4, 2000; 102(1): 14 - 20.
[Abstract] [Full Text] [PDF]

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 JAMAHome page
F. L. Brancati, W. H. L. Kao, A. R. Folsom, R. L. Watson, and M. Szklo
Incident Type 2 Diabetes Mellitus in African American and White Adults: The Atherosclerosis Risk in Communities Study
JAMA, May 3, 2000; 283(17): 2253 - 2259.
[Abstract] [Full Text] [PDF]

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 NEJMHome page
T. W. Gress, F. J. Nieto, E. Shahar, M. R. Wofford, F. L. Brancati, and The Atherosclerosis Risk in Communities Study
Hypertension and Antihypertensive Therapy as Risk Factors for Type 2 Diabetes Mellitus
N. Engl. J. Med., March 30, 2000; 342(13): 905 - 912.
[Abstract] [Full Text] [PDF]

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 J. Clin. Endocrinol. Metab.Home page
J.-M. Fernández-Real, M. Broch, J. Vendrell Cristóbal Richart, and W. Ricart
Interleukin-6 Gene Polymorphism and Lipid Abnormalities in Healthy Subjects
J. Clin. Endocrinol. Metab., March 1, 2000; 85(3): 1334 - 1339.
[Abstract] [Full Text]

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 Arterioscler. Thromb. Vasc. Bio.Home page
A. R. Folsom, K. K. Wu, M. Rasmussen, L. E. Chambless, N. Aleksic, and F. J. Nieto
Determinants of Population Changes in Fibrinogen and Factor VII Over 6 Years : The Atherosclerosis Risk in Communities (ARIC) Study
Arterioscler Thromb Vasc Biol, February 1, 2000; 20(2): 601 - 606.
[Abstract] [Full Text] [PDF]

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 J. Lipid Res.Home page
M. M. Sousa, L. Berglund, and M. J. Saraiva
Transthyretin in high density lipoproteins: association with apolipoprotein A-I
J. Lipid Res., January 1, 2000; 41(1): 58 - 65.
[Abstract] [Full Text]

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 J. Clin. Endocrinol. Metab.Home page
G. Paolisso, M. R. Tagliamonte, M. Barbieri, G. A. Zito, A. Gambardella, G. Varricchio, E. Ragno, and M. Varricchio
Chronic Vitamin E Administration Improves Brachial Reactivity and Increases Intracellular Magnesium Concentration in Type II Diabetic Patients
J. Clin. Endocrinol. Metab., January 1, 2000; 85(1): 109 - 115.
[Abstract] [Full Text]

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 J. Clin. Endocrinol. Metab.Home page
S. S. Damjanovic, M. S. Petakov, D. Micic, J. Marinkovic, C. Dieguez, F. F. Casanueva, and V. Popovic
Serum Leptin Levels in Patients with Acromegaly before and after Correction of Hypersomatotropism by Trans-Sphenoidal Surgery
J. Clin. Endocrinol. Metab., January 1, 2000; 85(1): 147 - 154.
[Abstract] [Full Text]

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 Arterioscler. Thromb. Vasc. Bio.Home page
M. E. Wittekoek, E. Moll, S. N. Pimstone, M. D. Trip, P. J. Lansberg, J. C. Defesche, J. J. van Doormaal, M. R. Hayden, and J. J. P. Kastelein
A Frequent Mutation in the Lipoprotein Lipase Gene (D9N) Deteriorates the Biochemical and Clinical Phenotype of Familial Hypercholesterolemia
Arterioscler Thromb Vasc Biol, November 1, 1999; 19(11): 2708 - 2713.
[Abstract] [Full Text] [PDF]

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 J. Biol. Chem.Home page
P. Sartipy, G. Camejo, L. Svensson, and E. Hurt-Camejo
Phospholipase A2 Modification of Low Density Lipoproteins Forms Small High Density Particles with Increased Affinity for Proteoglycans and Glycosaminoglycans
J. Biol. Chem., September 3, 1999; 274(36): 25913 - 25920.
[Abstract] [Full Text] [PDF]

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 J. Nutr.Home page
J. J. M. Castenmiller, C. E. West, J. P. H. Linssen, K. H. van het Hof, and A. G. J. Voragen
The Food Matrix of Spinach Is a Limiting Factor in Determining the Bioavailability of ß-Carotene and to a Lesser Extent of Lutein in Humans
J. Nutr., February 1, 1999; 129(2): 349 - 355.
[Abstract] [Full Text] [PDF]

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 Am. J. Clin. Nutr.Home page
M. S Alles, N. M de Roos, J C. Bakx, E. van de Lisdonk, P. L Zock, and J. G. Hautvast
Consumption of fructooligosaccharides does not favorably affect blood glucose and serum lipid concentrations in patients with type 2 diabetes
Am. J. Clinical Nutrition, January 1, 1999; 69(1): 64 - 69.
[Abstract] [Full Text] [PDF]

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 J. Clin. Endocrinol. Metab.Home page
N. Mekki, M. A. Christofilis, M. Charbonnier, C. Atlan-Gepner, C. Defoort, C. Juhel, P. Borel, H. Portugal, A. M. Pauli, B. Vialettes, et al.
Influence of Obesity and Body Fat Distribution on Postprandial Lipemia and Triglyceride-Rich Lipoproteins in Adult Women
J. Clin. Endocrinol. Metab., January 1, 1999; 84(1): 184 - 191.
[Abstract] [Full Text]

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 Arterioscler. Thromb. Vasc. Bio.Home page
P. Sartipy, G. Bondjers, and E. Hurt-Camejo
Phospholipase A2 Type II Binds to Extracellular Matrix Biglycan : Modulation of Its Activity on LDL by Colocalization in Glycosaminoglycan Matrixes
Arterioscler Thromb Vasc Biol, December 1, 1998; 18(12): 1934 - 1941.
[Abstract] [Full Text] [PDF]

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 J. Lipid Res.Home page
P. Borel, P. Grolier, N. Mekki, Y. Boirie, Y. Rochette, B. Le Roy, M. C. Alexandre-Gouabau, D. Lairon, and V. Azais-Braesco
Low and high responders to pharmacological doses of ß-carotene: proportion in the population, mechanisms involved and consequences on ß-carotene metabolism
J. Lipid Res., November 1, 1998; 39(11): 2250 - 2260.
[Abstract] [Full Text]

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 J. Nutr.Home page
Y. V. Yuan, D. D. Kitts, and D. V. Godin
Variations in Dietary Fat and Cholesterol Intakes Modify Antioxidant Status of SHR and WKY Rats
J. Nutr., October 1, 1998; 128(10): 1620 - 1630.
[Abstract] [Full Text]

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 CirculationHome page
A. R. Folsom, F. J. Nieto, P. Sorlie, L. E. Chambless, and D. Y. Graham
Helicobacter pylori Seropositivity and Coronary Heart Disease Incidence
Circulation, September 1, 1998; 98(9): 845 - 850.
[Abstract] [Full Text] [PDF]

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 J. Nutr.Home page
P. Borel, V. Tyssandier, N. Mekki, P. Grolier, Y. Rochette,, M. C. Alexandre-Gouabau, D. Lairon, and V. Azaïs-Braesco
Chylomicron beta -Carotene and Retinyl Palmitate Responses Are Dramatically Diminished When Men Ingest beta -Carotene with Medium-Chain Rather than Long-Chain Triglycerides
J. Nutr., August 1, 1998; 128(8): 1361 - 1367.
[Abstract] [Full Text]

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 CirculationHome page
A. R. Folsom, F. J. Nieto, P. G. McGovern, M. Y. Tsai, M. R. Malinow, J. H. Eckfeldt, D. L. Hess, and C. E. Davis
Prospective Study of Coronary Heart Disease Incidence in Relation to Fasting Total Homocysteine, Related Genetic Polymorphisms, and B Vitamins : The Atherosclerosis Risk in Communities (ARIC) Study
Circulation, July 21, 1998; 98(3): 204 - 210.
[Abstract] [Full Text] [PDF]

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 Arterioscler. Thromb. Vasc. Bio.Home page
F. Leyva, I. F. Godsland, M. Ghatei, A. J. Proudler, S. Aldis, C. Walton, S. Bloom, and J. C. Stevenson
Hyperleptinemia as a Component of a Metabolic Syndrome of Cardiovascular Risk
Arterioscler Thromb Vasc Biol, June 1, 1998; 18(6): 928 - 933.
[Abstract] [Full Text] [PDF]

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 J. Lipid Res.Home page
D. A. Bird, R. K. Tangirala, J. Fruebis, D. Steinberg, J. L. Witztum, and W. Palinski
Effect of probucol on LDL oxidation and atherosclerosis in LDL receptor-deficient mice
J. Lipid Res., May 1, 1998; 39(5): 1079 - 1090.
[Abstract] [Full Text] [PDF]

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 ANGIOLOGYHome page
F. Kosar, E. Varol, S. Ayaz, E. Kutuk, A. Oguzhan, and E. Diker
Plasma Leukocyte Elastase Concentration and Coronary Artery Disease
Angiology, March 1, 1998; 49(3): 193 - 201.
[Abstract] [PDF]

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 Arterioscler. Thromb. Vasc. Bio.Home page
F. Leyva, I. F. Godsland, M. Worthington, C. Walton, and J. C. Stevenson
Factors of the Metabolic Syndrome : Baseline Interrelationships in the First Follow-up Cohort of the HDDRISC Study (HDDRISC-1)
Arterioscler Thromb Vasc Biol, February 1, 1998; 18(2): 208 - 214.
[Abstract] [Full Text] [PDF]

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 Arterioscler. Thromb. Vasc. Bio.Home page
A. Ritsch, H. Drexel, F. W. Amann, C. Pfeifhofer, and J. R. Patsch
Deficiency of Cholesteryl Ester Transfer Protein : Description of the Molecular Defect and the Dissociation of Cholesteryl Ester and Triglyceride Transport in Plasma
Arterioscler Thromb Vasc Biol, December 1, 1997; 17(12): 3433 - 3441.
[Abstract] [Full Text]

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 J. Clin. Endocrinol. Metab.Home page
G. Targher, M. Alberiche, M. B. Zenere, R. C. Bonadonna, M. Muggeo, and E. Bonora
Cigarette Smoking and Insulin Resistance in Patients with Noninsulin-Dependent Diabetes Mellitus
J. Clin. Endocrinol. Metab., November 1, 1997; 82(11): 3619 - 3624.
[Abstract] [Full Text] [PDF]

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 CirculationHome page
A. R. Folsom, K. K. Wu, W. D. Rosamond, A. R. Sharrett, and L. E. Chambless
Prospective Study of Hemostatic Factors and Incidence of Coronary Heart Disease : The Atherosclerosis Risk in Communities (ARIC) Study
Circulation, August 19, 1997; 96(4): 1102 - 1108.
[Abstract] [Full Text]

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 J. Clin. Endocrinol. Metab.Home page
R. Sipilainen, M. Uusitupa, S. Heikkinen, A. Rissanen, and M. Laakso
Variants in the Human Intestinal Fatty Acid Binding Protein 2 Gene in Obese Subjects
J. Clin. Endocrinol. Metab., August 1, 1997; 82(8): 2629 - 2632.
[Abstract] [Full Text] [PDF]

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 J. Clin. Endocrinol. Metab.Home page
S. I. Sherman, M. D. Ringel, M. J. Smith, H. A. Kopelen, W. A. Zoghbi, and P. W. Ladenson
Augmented Hepatic and Skeletal Thyromimetic Effects of Tiratricol in Comparison with Levothyroxine
J. Clin. Endocrinol. Metab., July 1, 1997; 82(7): 2153 - 2158.
[Abstract] [Full Text] [PDF]

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 J. Clin. Endocrinol. Metab.Home page
G. Paolisso, S. Ammendola, A. Del Buono, A. Gambardella, M. Riondino, M. R. Tagliamonte, M. R. Rizzo, C. Carella, and M. Varricchio
Serum Levels of Insulin-Like Growth Factor-I (IGF-I) and IGF-Binding Protein-3 in Healthy Centenarians: Relationship with Plasma Leptin and Lipid Concentrations, Insulin Action, and Cognitive Function
J. Clin. Endocrinol. Metab., July 1, 1997; 82(7): 2204 - 2209.
[Abstract] [Full Text] [PDF]


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