Clinical Chemistry
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Clinical Chemistry 29: 1104-1105, 1983;
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Clinical Chemistry, Vol 29, 1104-1105, Copyright © 1983 by American Association for Clinical Chemistry

Liquid-chromatographic determination of aminoglutethimide in plasma

BA Robinson and FN Cornell

A simple, rapid "high-performance" liquid-chromatographic procedure is presented for the determination of aminoglutethimide in plasma. After precipitation of the protein with acetonitrile, an aliquot of the supernate is injected directly onto a radially compressed, reversed- phase column. The aminoglutethimide is isocratically eluted with a mobile phase of acetonitrile/water/tert-butyl ammonium phosphate. The method is both accurate and precise and has been in routine use in our laboratory for more than 12 months.




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Copyright © 1983 by the American Association for Clinical Chemistry.