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Clinical Chemistry 29: 1385-1388, 1983;
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Clinical Chemistry, Vol 29, 1385-1388, Copyright © 1983 by American Association for Clinical Chemistry

Immunonephelometry of apolipoprotein A-II in hyperlipoproteinemic serum

CC Heuck, I Erbe, K Frech, P Lohse and G Munscher

Immunonephelometry of apolipoprotein A-II (apo A-II) in serum requires that factors responsible for nonspecific increases of light scattering be eliminated. Complete delipidation is not necessary for immunonephelometry of apo A-II; in fact, light scattering of immunocomplexes from delipidated HDL is greater than from isolated apo A-II. Therefore, using the isolated peptide as a standard does not suffice for measurements in serum. The concentration of protein influences the development of light scattering and must be considered in standardizing this measurement.




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Copyright © 1983 by the American Association for Clinical Chemistry.