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Clinical Chemistry 29: 1399-1403, 1983;
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Clinical Chemistry, Vol 29, 1399-1403, Copyright © 1983 by American Association for Clinical Chemistry

Sensitive colorimetric assay for angiotensin converting enzyme in serum

HM Neels, ME van Sande and SL Scharpe

A sensitive colorimetric procedure has been developed for the assay of angiotensin converting enzyme (EC 3.4.15.1) in serum. Serum (10 microL) is incubated for 30 min with hippuryl-glycyl-glycine as described earlier (Clin Chem 28: 1352-1355, 1982). After a Folin-Wu deproteinization, the liberated glycyl-glycine is derivatized with a borate-buffered (pH 9.3) trinitrobenzenesulfonate solution (60 mmol/L) to form trinitrophenyl-glycylglycine, the absorbance of which is read at 420 nm vs a serum blank. The linear range extends to an activity of more than 900 U/L of serum and the detection limit is less than 4 U/L. The mean activity for serum from 50 blood bank donors and 25 patients with active sarcoidosis was 281 (SD 77) and 693 (SD 81) U/L, respectively. The method demonstrates good precision (CVs less than 2.8%) and correlates well (r = .99) with results from a "high-pressure" liquid chromatographic procedure for determining hippuric acid. In addition, the proposed method is widely applicable, involving only commonly available apparatus.


The following articles in journals at HighWire Press have cited this article:


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H. Wang, M. J. Katovich, C. H. Gelband, P. Y. Reaves, M. I. Phillips, and M. K. Raizada
Sustained Inhibition of Angiotensin I-Converting Enzyme (ACE) Expression and Long-Term Antihypertensive Action by Virally Mediated Delivery of ACE Antisense cDNA
Circ. Res., October 1, 1999; 85(7): 614 - 622.
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Copyright © 1983 by the American Association for Clinical Chemistry.