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Clinical Chemistry 29: 1508-1512, 1983;
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Clinical Chemistry, Vol 29, 1508-1512, Copyright © 1983 by American Association for Clinical Chemistry

Potentiometry of Na+ in undiluted serum and urine with use of an improved neutral carrier-based solvent polymeric membrane electrode

P Anker, HB Jenny, U Wuthier, R Asper, D Ammann and W Simon

We present an improved Na+-selective liquid membrane electrode for measurement of Na+ concentrations in both undiluted serum and urine. The values for urinary Na+ obtained with the ion-selective electrode agree well with those obtained with the flame photometer. The correlation gives a standard residual deviation of +/- 2.7 mmol/L over the Na+ range of 25-280 mmol/L. In serum, this direct potentiometry yields Na+ concentrations 5.4% (SD 1.1%) higher than those obtained by atomic spectrometry and a standard residual deviation of +/- 1.1 mmol/L. Correction of these values for the volumes of protein and lipid leads to potentiometric values 1.2% (SD 0.7%) lower than those by flame photometry (residual standard deviation: +/- 1.0 mmol/L). Other factors that possibly contribute to this discrepancy are discussed.


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D. E. Carden, D. Diamond, and A. J. Miller
An improved Na+-selective microelectrode for intracellular measurements in plant cells
J. Exp. Bot., June 1, 2001; 52(359): 1353 - 1359.
[Abstract] [Full Text] [PDF]


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Copyright © 1983 by the American Association for Clinical Chemistry.