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Clinical Chemistry 29: 1540-1542, 1983;
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Clinical Chemistry, Vol 29, 1540-1542, Copyright © 1983 by American Association for Clinical Chemistry

Quantitation of haptens by homogeneous immunoprecipitation. 2. Centrifugal analysis for tobramycin, phenobarbital, and theophylline in serum

JW Wu, C Bunyagidj, S Hoskin, SM Riebe, J Aucker, K White and SP O'Neill

Our homogeneous immunoprecipitation inhibition assay (Clin. Chem. 28:659-661, 1982) is applied here to tobramycin, phenobarbital, and theophylline. Only 10-50 microL of test sample is needed. No sample treatment, dilution, or extraction is required. A test serum sample is simultaneously mixed with a drug conjugate and its specific antiserum in a centrifugal analyzer. The subsequent reaction and the measurement are completed in 3 min. Within-run and between-run CVs for clinically relevant concentrations were well below 10%. Results for patients' samples correlated well with those by enzyme immunoassay.




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Copyright © 1983 by the American Association for Clinical Chemistry.