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Clinical Chemistry 29: 1543-1546, 1983;
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Clinical Chemistry, Vol 29, 1543-1546, Copyright © 1983 by American Association for Clinical Chemistry

Simultaneous liquid-chromatographic determination of hypoxanthine, xanthine, urate, and creatinine in cerebrospinal fluid, with direct injection

F Niklasson

In this method for simultaneously determining hypoxanthine, xanthine, urate, and creatinine in cerebrospinal fluid, centrifuged sample is directly injected on a reversed-phase liquid-chromatographic column. On elution with potassium phosphate buffer the compounds are quantified by their absorbance at 260 nm. Random error (CV) was between 1.2 and 3.4% and analytical recoveries were 99-104% at physiological concentrations.




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Copyright © 1983 by the American Association for Clinical Chemistry.