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Clinical Chemistry 29: 1582-1586, 1983;
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Clinical Chemistry, Vol 29, 1582-1586, Copyright © 1983 by American Association for Clinical Chemistry

Immunoassay techniques with fluorescent phycobiliprotein conjugates

MN Kronick and PD Grossman

We describe immunoassay techniques that take advantage of the novel properties of phycobiliprotein fluorescent dyes. These dyes, which can be isolated from a wide variety of algae, exhibit extremely high absorptivities, high quantum efficiencies, and excitation and emission bands across the visible spectrum. These stable, hydrophilic proteins can easily be linked to antibodies by conventional protein cross- linking reagents. One assay technique presented here demonstrates the use of phycobiliproteins in a solid-phase "sandwich" assay, which achieves picomolar sensitivity. In the other technique the unique spectral properties of phycobiliproteins are applied to fluorescence excitation transfer immunoassay.


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Copyright © 1983 by the American Association for Clinical Chemistry.