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Clinical Chemistry 29: 1635-1640, 1983;
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Clinical Chemistry, Vol 29, 1635-1640, Copyright © 1983 by American Association for Clinical Chemistry

Applications of fast protein liquid chromatography TM in the separation of plasma proteins in urine and cerebrospinal fluid

EH Cooper, R Turner, EA Johns, H Lindblom and VJ Britton

Fast Protein Liquid Chromatography TM (FPLC), in which an anion- exchange column is used, provides rapid separation and reproducible profiling of the plasma proteins in urine and cerebrospinal fluid (CSF). Chromatographic separation of the proteins takes 1 h for urine specimens and 45 min for CSF. The elution sequence from the anion- exchange column is similar to the electrophoretic mobility. Individual proteins have the same retention times independently of which type of specimen is used. The elution characteristics of 21 plasma proteins have been identified. We illustrated some applications of this system, including the profiling of tubular protein-uria, the isolation of Bence Jones proteins from urine, and the investigation of hemoglobin-derived products in the CSF.




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Copyright © 1983 by the American Association for Clinical Chemistry.