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Clinical Chemistry 3: 169-177, 1957;
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Clinical Chemistry, Vol 3, 169-177, Copyright © 1957 by the American Association for Clinical Chemistry

Measurement of Uric Acid in Biologic Fluids Using an Ion-Exchange Separation

Bernard Shapiro 1, David Seligson 1, and Ralph Jessar 1

1 Chemical Section, Department of Medicine, Hospital of the University of Pennsylvania, the Division of Biochemistry of the Graduate Hospital, and the Department of Medicine of the Graduate School of Medicine, University of Pennsylvania, Philadelphia, Pa.

A new method for the measurement of uric acid in biologic fluids is presented. It involves the isolation of uric acid by ion-exchange separation and quantitative determination of uric acid in the eluate by ultraviolet absorption, the Folin-Brown method or an enzymatic technic. The enzymatic determination on the eluate is the most specific of the technics.

A series of determinations of uric acid in serum compares well with determinations on the same samples using the direct Folin-Brown procedure. The direct Folin-Brown method appears to be in error in determinations on certain urine samples. Measurements of uric acid in bile are reported. The technic can be used in the routine clinical laboratory, and is particularly applicable to fluids which cannot be studied by direct methods.

Submitted on March 1, 1957






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Copyright © 1957 by the American Association for Clinical Chemistry.