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Clinical Chemistry 3: 711-715, 1957;
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Clinical Chemistry, Vol 3, 711-715, Copyright © 1957 by the American Association for Clinical Chemistry

Absorbance of Various Protein-Free Filtrates of Serum

Richard J. Henry 1 and Sam Berkman 1

1 Bio-Science Laboratories, 2231 S. Carmelina Ave., Los Angeles, Calif.

Absorbance curves have been run on protein-free serum filtrates obtained with trichloroacetic acid, tungstic acid, and zinc hydroxide. The background absorbance was highest in trichloroacetic acid filtrates, lowest in zinc hydroxide filtrates, and increased with decreasing wavelength. The possible error produced in photometric determinations by this nonspecific absorbance and means of correction have been discussed.

Submitted on September 23, 1957






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Copyright © 1957 by the American Association for Clinical Chemistry.