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Clinical Chemistry 3: 716-727, 1957;
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Clinical Chemistry, Vol 3, 716-727, Copyright © 1957 by the American Association for Clinical Chemistry

Studies with a New Colorimetric Test for Proteinuria

Alfred H. Free 1, Chauncey O. Rupe 1, and Ingrid Mefzler 1

1 Miles-Ames Research Laboratory, Elkhart, Ind.

A color reaction of protein has been formulated into two simple colorimetric tests for protein in urine; one is a tablet test, consisting of a salicylate buffer and bromphenol blue, and the other is a strip test, consisting of a paper strip impregnated with a citrate buffer and tetrabromphenol blue. The basic principle in both tests is that of "protein error of indicators." Experiments with approximately 5000 urines, taken from both hospital patients and normal subjects, reveal that these tests are simple, rapid, accurate, sensitive, specific, and capable of being used with turbid urines.

Submitted on July 19, 1957




The following articles in journals at HighWire Press have cited this article:


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J. Barratt MB ChB (Ho and P. Topham MB ChB MD
Urine proteomics: the present and future of measuring urinary protein components in disease
Can. Med. Assoc. J., August 14, 2007; 177(4): 361 - 368.
[Abstract] [Full Text] [PDF]


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Copyright © 1957 by the American Association for Clinical Chemistry.