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Clinical Chemistry 30: 111-113, 1984;
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Clinical Chemistry, Vol 30, 111-113, Copyright © 1984 by American Association for Clinical Chemistry

Pyruvate kinase isoenzymes in human serum

JL Ngo, KN Garratt, P Eversole, RA Orlando and KH Ibsen

Pyruvate kinase activity in serum from presumably healthy individuals is labile, but can be maintained for a week when samples are stored frozen. Contrary to published data our electrophoretic and isoelectrofocusing studies with fresh sera show the presence of both K and M isoenzymes; the latter predominates. In freezer-stored serum the putative M isoenzyme showed a progressive decrease in pl value as well as a decreased electrophoretic mobility.





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Copyright © 1984 by the American Association for Clinical Chemistry.