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Clinical Chemistry 30: 131-135, 1984;
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Clinical Chemistry, Vol 30, 131-135, Copyright © 1984 by American Association for Clinical Chemistry

Simplified determination of serotonin in plasma by liquid chromatography with electrochemical detection

PC Tagari, DJ Boullin and CL Davies

This simplified determination of serotonin in human plasma involves "high-performance" liquid chromatography with electrochemical detection. Deproteinated samples of platelet-poor plasma are injected directly into the chromatograph without preliminary chromatographic purification. The limit of quantitative detection of serotonin in plasma is 5 nmol/L (equivalent to 250 fmol injected). Intra- and inter- assay CVs ranged from 5.6% to 7.7% and 3.5% to 14.9%, respectively, for concentrations of serotonin from 0.02 to 10.0 mumol/L. Results agreed well with those by spectrofluorometry, both for plasma standards (r = 0.985, range 100 nmol/L to 100 mumol/L) and for pathological samples obtained from four carcinoid syndrome patients (r = 0.964). The method is also applicable to the concurrent measurement of 5-hydroxytryptophan and 5-hydroxyindoleacetic acid.


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Copyright © 1984 by the American Association for Clinical Chemistry.