Clinical Chemistry
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Clinical Chemistry 30: 140-143, 1984;
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Clinical Chemistry, Vol 30, 140-143, Copyright © 1984 by American Association for Clinical Chemistry

Improved liquid-chromatographic determination of 3-methoxy-4- hydroxyphenylethyleneglycol in urine with electrochemical detection

JR Shipe, J Savory and MR Wills

In this improved method for quantifying 3-methoxy-4- hydroxyphenylethyleneglycol (MHPG) in urine, after a multistep extraction of MHPG and internal standard (iso-MHPG) from 3.0 mL of urine, the compounds are separated on a C18 reversed-phase column and quantified by use of an electro-chemical detector. The isocratic chromatographic separation takes about 16 min. The mobile phase is phosphate buffer/acetonitrile (88/12 by vol), the flow rate 0.7 mL/min. Recycling the mobile phase and automating the sample injection make possible the unattended assay of more than 70 samples per day. The within-run precision of the method is excellent (CV 1.8%) at a mean concentration of 1.1 mg/L.




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Copyright © 1984 by the American Association for Clinical Chemistry.