Clinical Chemistry
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Clinical Chemistry 30: 98-100, 1984;
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Clinical Chemistry, Vol 30, 98-100, Copyright © 1984 by American Association for Clinical Chemistry

Noncompetitive enzyme-linked immunoassay for apolipoprotein B in serum

C Fievet, M Koffigan, D Ouvry, S Marcovina, Y Moschetto and JC Fruchart

We used a noncompetitive enzyme-linked immunoassay to measure apolipoprotein B (apo-B) concentration in human plasma. Goat anti- lipoprotein B immunoglobulins were adsorbed to the surface of polystyrene balls. After washing, this solid-phase antibody was incubated with antigen (plasma from normal or hyperlipoproteinemic fasting subjects), washed, and then incubated with peroxidase-labeled goat anti-lipoprotein B IgG. After a last washing, we measured the bound label, which provided a direct measurement of the antigen. Under optimized assay conditions, the minimum detectable concentration was 50 ng per assay. The assay may be used to measure apo-B in different lipoprotein fractions (low- or very-low-density) and yields values that compared favorably with those obtained by electroimmunoassay (r = 0.86). The assay offers several advantages over existing techniques: sensitivity, specificity, simplicity, avoidance of radioisotopes, and potential for use with monoclonal antibodies.


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 Clin. Chem.Home page
F. Ferrer, E. Bigot-Corbel, P. N'Guyen, M. Krempf, and J.-M. Bard
Quantitative Measurement of Lipoprotein Particles Containing Both Apolipoprotein AIV and Apolipoprotein B in Human Plasma by a Noncompetitive ELISA
Clin. Chem., June 1, 2002; 48(6): 884 - 890.
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Copyright © 1984 by the American Association for Clinical Chemistry.