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Clinical Chemistry 30: 1674-1676, 1984;
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Clinical Chemistry, Vol 30, 1674-1676, Copyright © 1984 by American Association for Clinical Chemistry

Silver-stained phenotyping of alpha 1-antitrypsin in dried blood and serum specimens

G Massi, G Marano, F Patalano and P Auconi

In this technique for determining the electrophoretic phenotype of alpha 1-antitrypsin in dried blood or serum specimens, the adsorbed material is eluted with a concentrated solution of dithiothreitol, focused on polyacrylamide thin-layer gel, and made visible with silver stain. With this staining technique all normal and pathological alpha 1- antitrypsin phenotypes can be detected. The procedure is relatively simple, inexpensive, and suitable for use in large-scale screening for alpha 1-antitrypsin deficiency in selected populations.


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Copyright © 1984 by the American Association for Clinical Chemistry.