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Clinical Chemistry 30: 1746-1752, 1984;
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Clinical Chemistry, Vol 30, 1746-1752, Copyright © 1984 by American Association for Clinical Chemistry

An automated "high-pressure" liquid-chromatographic assay for hemoglobin A1c

G Ellis, EP Diamandis, EE Giesbrecht, D Daneman and LC Allen

An automated "high-pressure" liquid-chromatographic assay for hemoglobin A1c is described. We use a 45-min incubation in acetate buffer (pH 5.5) to eliminate labile glycated hemoglobins. In this automated system conventional modules are used but it incorporates a solvent-switching valve to select either of two buffers, which differ in pH and NaCl concentration. The chromatographic column contains "polyCAT" (a weak cation-exchanger, polyaspartic acid linked to silica). Run time is 14 min per sample. The method is precise and results correlate well with those by other ion-exchange procedures.


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