Clinical Chemistry
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Clinical Chemistry 30: 1753-1757, 1984;
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Clinical Chemistry, Vol 30, 1753-1757, Copyright © 1984 by American Association for Clinical Chemistry

New photometric assay for chymotrypsin in stool

P Kaspar, G Moller and A Wahlefeld

In this new photometric assay fecal samples are pretreated with detergent and high concentrations of salts. The subsequent kinetic enzyme determination step involves the chromogenic substrate succinyl- Ala-Ala-Pro-Phe-4-nitroanilide. The sample pretreatment assures a nearly complete solubilization of the formerly particle-bound enzyme, thus permitting determination of the enzyme activity in either the suspension or the supernate after centrifugation. Furthermore this pretreatment enhances the enzyme activity and decreases the Km value. Results by this assay correlate well with those by classical titrimetry and the method is easily adapted to automated systems.


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J. Walkowiak, K.-H. Herzig, K. Strzykala, J. Przyslawski, and M. Krawczynski
Fecal Elastase-1 Is Superior to Fecal Chymotrypsin in the Assessment of Pancreatic Involvement in Cystic Fibrosis
Pediatrics, July 1, 2002; 110(1): e7 - 7.
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Copyright © 1984 by the American Association for Clinical Chemistry.