Clinical Chemistry
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Clinical Chemistry 30: 1770-1773, 1984;
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Clinical Chemistry, Vol 30, 1770-1773, Copyright © 1984 by American Association for Clinical Chemistry

Determination of free thyroxin in serum by ultrafiltration: validation of a method and preliminary results

N Shannon and PD Woolf

We describe the determination of concentrations of free thyroxin in serum by ultrafiltration based on the Amicon micropartition system. The assay is straightforward and requires about 4 h for 25 samples. Our results demonstrate a correlation coefficient of 0.81 compared with equilibrium dialysis, and of 0.88 compared with radioimmunoassay. For sera from 49 normal, 25 hyperthyroid, 24 hypothyroid, 50 euthyroid sick, and 13 pregnant subjects, the means (and SD) were respectively: 19.3 (4.9), 40.1 (13.6), 5.0 (2.9), 25.9 (10.5), and 16.1 (3.2) ng/L. The interassay CV was 9.9% and the intra-assay CV 7.2%. We conclude that this procedure is useful in the diagnosis of thyroid disease and offers advantages of simplicity, rapidity, and economy.


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N. D. Christofides, E. Wilkinson, M. Stoddart, D. C. Ray, and G. J. Beckett
Assessment of Serum Thyroxine Binding Capacity-dependent Biases in Free Thyroxine Assays
Clin. Chem., April 1, 1999; 45(4): 520 - 525.
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