Clinical Chemistry
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Clinical Chemistry 30: 1780-1783, 1984;
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Clinical Chemistry, Vol 30, 1780-1783, Copyright © 1984 by American Association for Clinical Chemistry

Determination of enzyme activity in biological fluids by means of electrochemical oxidation of NADH at a modified glassy carbon electrode

L Bartalits, G Nagy and E Pungor

This amperometric technique for the determination of enzyme activity is based on detecting a decrease in the concentration of the NADH co- factor of the enzyme reaction. A glassy carbon electrode, modified by adsorption of Mg2+ and NADH, is used to measure the anodic peak current that corresponds to the oxidation of NADH. We found no significant difference between the enzyme activity of lactate dehydrogenase (E.C.1.1.1.27) preparations as measured by the above amperometric technique and by a spectrophotometric method.




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Copyright © 1984 by the American Association for Clinical Chemistry.