Clinical Chemistry Siemens Point of Care - Urinalysis
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Clinical Chemistry 30: 1789-1791, 1984;
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Clinical Chemistry, Vol 30, 1789-1791, Copyright © 1984 by American Association for Clinical Chemistry

Measurement of gliclazide in plasma by radial compression reversed- phase liquid chromatography

BG Charles and PJ Ravenscroft

We describe a rapid, specific, and precise analysis for gliclazide in plasma by radial compression, reversed-phase, "high-performance" liquid chromatography. Gliclazide and the internal standard, 3- chlorogliclazide, are eluted after 4.4 and 6.8 min, respectively. Only 100 microL of plasma and minimal sample workup are required. The limit of detection for gliclazide in plasma is 0.5 mg/L (1.55 mumol/L) at 229 nm. Precision (CV) of the assay for 10 and 1 mg of gliclazide per litre is 2.1% and 6.4%, respectively.




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Copyright © 1984 by the American Association for Clinical Chemistry.