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Clinical Chemistry 30: 1801-1804, 1984;
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Clinical Chemistry, Vol 30, 1801-1804, Copyright © 1984 by American Association for Clinical Chemistry

Automated calmagite compleximetric measurement of magnesium in serum, with sequential addition of EDTA to eliminate endogenous interference

RJ Liedtke and G Kroon

We describe an automated method for magnesium assay, for use with the Cobas-Bio centrifugal analyzer. Magnesium is reacted with calmagite, a dye, and the absorbance of the magnesium-dye complex at 520 nm is measured. EDTA is then added to break up the complex and the absorbance at 520 nm is re-measured to correct for sample color and turbidity. Including ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'- tetraacetic acid (EGTA) and triethanolamine in the dye reagent eliminates interference by calcium and iron. Within-run CVs were less than 2.0% for concentrations of magnesium ranging from 18 to 40 mg/L, and less than 4% for a magnesium concentration of 9.4 mg/L. Day-to-day precision data, determined over five months were: mean = 21.0 mg/L, CV = 2.7%; mean = 43.0 mg/L, CV = 3.2% (n = 550 for both). Comparison of the Cobas-Bio method (y) with an atomic absorption spectrometric method (x) gave the following results: y = 0.968x + 0.448, r = 0.986, Sy/x = 0.34 mg/L, mean x = 19.8 mg/L, mean y = 19.6 mg/L, n = 44. Hemoglobin, bilirubin, and turbidity do not interfere. The standard curve is linear up to a magnesium concentration of 97 mg/L.


The following articles in journals at HighWire Press have cited this article:


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 Annals of Clinical & Laboratory ScienceHome page
H. S. Aziz, A. I. Blamoun, M. K. Shubair, M. M.F. Ismail, V. A. DeBari, and M. A. Khan
Serum Magnesium Levels and Acute Exacerbation of Chronic Obstructive Pulmonary Disease: A Retrospective Study
Ann. Clin. Lab. Sci., October 1, 2005; 35(4): 423 - 427.
[Abstract] [Full Text] [PDF]


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