Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 30: 1809-1811, 1984;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Leflar, C. C.
Right arrow Articles by Miller, W. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Leflar, C. C.
Right arrow Articles by Miller, W. K.

Clinical Chemistry, Vol 30, 1809-1811, Copyright © 1984 by American Association for Clinical Chemistry

An automated, affinity-column-mediated, enzyme-linked immunometric assay for digoxin on the Du Pont aca discrete clinical analyzer

CC Leflar, JW Freytag, LM Powell, JC Strahan, JJ Wadsley, CA Tyler and WK Miller

We describe an automated assay for digoxin that requires a 200-microL sample of serum. Total analysis time is 18 min. The method is extremely precise, with within-run CVs of 2.6, 1.6, and 4.9%, respectively, at 0.5, 1.5, and 3.5 micrograms of digoxin per liter (n = 20). The lower limit of detection is 0.2 micrograms of digoxin per liter. For patients' samples, the correlation with RIA (x) is excellent (r = 0.95; y = 0.95x - 0.14; standard error = 0.17 micrograms/mL). We saw no interferences in samples having high concentrations of rheumatoid factors, lipid, bilirubin, or hemoglobin. Cross reactivity with digoxin analogs and steroidal compounds is similar to that observed by RIA.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1984 by the American Association for Clinical Chemistry.