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Clinical Chemistry 30: 1812-1814, 1984;
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Clinical Chemistry, Vol 30, 1812-1814, Copyright © 1984 by American Association for Clinical Chemistry

Effects of sample preparation on concentrations of cyclosporin A measured in plasma

RW Yatscoff, DN Rush and JR Jeffery

Because cyclosporin A rapidly changes its distribution in blood with changes in temperature, sample preparation affects results for it as measured in plasma. If whole blood is stored at either 4 degrees C or room temperature, results for cyclosporin A in the plasma are lower than in whole blood stored at 37 degrees C and centrifuged at this temperature. Re-equilibration of the former to 37 degrees C before cells are removed increases the analytical recovery of cyclosporin A in plasma; the optimal equilibration interval is 30 min. Use of such re- equilibration, followed by immediate centrifugation at room temperature, increases values obtained for cyclosporin in plasma by 60 to 65% over those determined after non-temperature-standardized collection procedures, but does not significantly improve the correlation between values for plasma and whole blood. Hematocrit and concentrations of cyclosporin A in plasma are inversely related. Correction for hematocrit improves the correlation between results for plasma and whole blood.


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A. R. Terrell, T. M. Daly, K. G. Hock, D. C. Kilgore, T. Q. Wei, S. Hernandez, D. Weibe, L. Fields, L. M. Shaw, and M. G. Scott
Evaluation of a No-Pretreatment Cyclosporin A Assay on the Dade Behring Dimension RxL Clinical Chemistry Analyzer
Clin. Chem., July 1, 2002; 48(7): 1059 - 1065.
[Abstract] [Full Text] [PDF]


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Copyright © 1984 by the American Association for Clinical Chemistry.