Clinical Chemistry
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Clinical Chemistry 30: 1821-1824, 1984;
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Clinical Chemistry, Vol 30, 1821-1824, Copyright © 1984 by American Association for Clinical Chemistry

Automated kinetic assay for lactate dehydrogenase isoenzyme 1 by centrifugal analysis after alkaline inactivation

JH Eckfeldt, MJ Kershaw and LA Lewis

Lactate dehydrogenase isoenzyme 1 (LD1, EC 1.1.1.27) has been used widely as a marker for myocardial infarction, and many analytical methods for it have been developed, most of which are relatively labor intensive. On the basis of a recent report by Takizawa et al. (Clin Chem 29: 1941-1945, 1983) describing the marked stability of LD1 in buffered alkaline solutions, and with use of a centrifugal analyzer, we have developed a fully automated assay for LD1. Results with this method are precise (between-day SD = 2.1 U/L), vary linearly with LD1 activity to 600 U/L, and correlate well with LD1 as determined by immunological (Roche Isomune-LD) and agarose electrophoretic methods (r = 0.985 and 0.986, respectively). Furthermore, the method is easy and convenient, and should provide a substantial savings in labor and reagent costs to laboratories currently determining LD1 by electrophoretic or immunologic methods.




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Copyright © 1984 by the American Association for Clinical Chemistry.