Clinical Chemistry
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Clinical Chemistry 30: 1834-1835, 1984;
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Clinical Chemistry, Vol 30, 1834-1835, Copyright © 1984 by American Association for Clinical Chemistry

Nephelometry of acute-phase glycoproteins by binding to concanavalin A

EM Pitkanen, T Palosuo, K Aho, T Putus-Tikkanen and R von Essen

Nephelometry of serum acute-phase glycoproteins by binding to concanavalin A (con-A) was compared with assays for haptoglobin and alpha 1-acid glycoprotein, and for C-reactive protein. The cutoff points for positive reactions were determined on the basis of results for a random sample (n = 130) from a middle-aged population. The sensitivity of the con-A binding assay compared favorably with that of individual acute-phase glycoproteins in a follow-up cohort of 198 patients with inflammatory joint diseases. Unlike the case in many individual acute-phase glycoprotein assays, the distribution of con-A binding values in healthy subjects is remarkably symmetrical, allowing an easy distinction between abnormal and normal values.




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Copyright © 1984 by the American Association for Clinical Chemistry.