Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 30: 1845-1847, 1984;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Levin, K.
Right arrow Articles by Engstrom, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Levin, K.
Right arrow Articles by Engstrom, I.

Clinical Chemistry, Vol 30, 1845-1847, Copyright © 1984 by American Association for Clinical Chemistry

Inadequate hemolysis of erythrocytes on reagent strips at low pH causes false-negative readings

K Levin and I Engstrom

Patients' urines gave an unexpectedly high percentage of false-negative results for erythrocytes on a reagent strip, even when the test field for ascorbic acid indicated that none of this substance was present to inhibit the test reaction. By adding known amounts of erythrocytes to normal urines, we found that this phenomenon was time-dependent and occurred with three different brands of reagent strips in urines having a pH below 6.0. Hemolysis of erythrocytes was totally inhibited below pH 5.1 after 4 h of incubation with 50 X 10(6) erythrocytes per liter. The inhibition is apparently caused by shrinkage of the erythrocytes, rending them insensitive to the hemolytic agents in the different reagent strips tested.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1984 by the American Association for Clinical Chemistry.