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Clinical Chemistry 30: 1848-1850, 1984;
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Clinical Chemistry, Vol 30, 1848-1850, Copyright © 1984 by American Association for Clinical Chemistry

Enzyme immunoassay for pregnancy-associated plasma protein-A

DJ MacDonald, HC Mehta, DS Mack, T Duffy and AC Glen

This enzyme immunoassay procedure for pregnancy-associated plasma protein type A involves a "sandwich"-type system in microtitration plates. One can detect 0.27 mg of the protein per liter of serum, with a between-batch CV of 10.2%, and the antiserum used does not cross react with any of the other placentally derived proteins. A reference interval for the last trimester of pregnancy is presented. The procedure described is suitable for studying the behavior of this protein during pregnancy.


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Q.-P. Qin, M. Christiansen, C. Oxvig, K. Pettersson, L. Sottrup-Jensen, C. Koch, and B. Norgaard-Pedersen
Double-monoclonal immunofluorometric assays for pregnancy-associated plasma protein A/proeosinophil major basic protein (PAPP-A/proMBP) complex in first-trimester maternal serum screening for Down syndrome
Clin. Chem., December 1, 1997; 43(12): 2323 - 2332.
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Copyright © 1984 by the American Association for Clinical Chemistry.