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Clinical Chemistry 30: 1947-1949, 1984;
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Clinical Chemistry, Vol 30, 1947-1949, Copyright © 1984 by American Association for Clinical Chemistry

Two-dimensional electrophoretic analysis of cytosols from human breast tumors: optimal migration conditions

P Motte, JM Bidart, JC Delarue, E Comoy, P Moingeon and C Bohuon

In an examination of cytosols from human breast tumors, we performed two-dimensional electrophoretic analysis. Several migration conditions were tried in a search for a homogeneous repartition of cytosolic proteins. The most workable gels were obtained with a 4.40 to 8.05 pH gradient in the first dimension and a homogeneous 125 g/L acrylamide/sodium dodecyl sulfate gel, 1 mm in thickness, in the second dimension. The practicability of this method is discussed.





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Copyright © 1984 by the American Association for Clinical Chemistry.