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Clinical Chemistry 30: 2084-2092, 1984;
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Clinical Chemistry, Vol 30, 2084-2092, Copyright © 1984 by American Association for Clinical Chemistry

Two-dimensional electrophoresis of human plasma apolipoproteins

DL Sprecher, L Taam and HB Brewer Jr

The two-dimensional electrophoretic method with silver staining we describe better resolves plasma apolipoproteins (apo) than any procedure previously described. It can be used to screen for abnormalities in apoA-I, apoA-II, apoA-IV, apoC-II, apoC-III, apoD, apoE, and apoH. In addition, this is the first presentation of apoD and apoH on two-dimensional gels. This electrophoretic method will permit the quantification of plasma apolipoproteins by computer imaging. Such detailed analysis of the plasma apolipoproteins should provide a better understanding of apolipoprotein function and the role these apolipoproteins play in lipoprotein metabolism and the pathophysiology of dyslipoproteinemias and coronary artery disease.


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