Clinical Chemistry
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Clinical Chemistry 30: 284-286, 1984;
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Clinical Chemistry, Vol 30, 284-286, Copyright © 1984 by American Association for Clinical Chemistry

Evaluation of a direct solid-phase radioimmunoassay for progesterone, useful for monitoring luteal function

NP Kubasik, GD Hallauer and RG Brodows

We have evaluated a commercially available, direct, solid-phase radioimmunoassay kit for progesterone determination in serum or plasma. The assay is precise, within-run precision (CV) in the clinically significant ranges being 2.5 to 5.2%, between-run 5.5 to 5.8%. Mean analytical recovery of different concentrations of progesterone added to serum was 99.7% (range 95.3 to 102.7%). Fourteen closely related steroids showed no cross reactivity. The minimum detection limit was 0.5 microgram/L. Luteal-phase progesterone concentrations in serum were increased (greater than 3 micrograms/L) in 19 normal ovulatory menstrual cycles and decreased (less than 1.5 micrograms/L) in two nonovulatory cycles. We found this direct assay for progesterone to be analytically and clinically sound, and useful for assessing luteal- phase function.


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Copyright © 1984 by the American Association for Clinical Chemistry.