Clinical Chemistry
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Clinical Chemistry 30: 287-290, 1984;
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Clinical Chemistry, Vol 30, 287-290, Copyright © 1984 by American Association for Clinical Chemistry

Dual-channel continuous-flow system for determination of phenylalanine and galactose: application to newborn screening

GL Hoffman, RH Laessig, DJ Hassemer and ER Makowski

We describe a dual-channel AutoAnalyzer (Technicon) system for the simultaneous measurement of phenylalanine and galactose from blood specimens on filter-paper. Using a single 1/4-in.-diameter (6-mm) specimen, we measure both components fluorometrically at a rate of 70/h. Analytical recovery with the method and the linearity of measurements vs sample concentration are excellent through the ranges of interest, 0-200 mg/L for phenylalanine and 0-800 mg/L for galactose. Carryover at the critical values during screening, 40 mg/L for phenylalanine and 100 mg/L for galactose, is essentially zero. The dual- channel system provides the means to incorporate a low-incidence test, i.e., galactosemia (incidence 1/70 000), into an existing program for phenylalanine analysis, for which the higher rates (phenylketonuria, incidence 1/11 500) easily justify the cost of mass screening.


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 Clin. Chem.Home page
A. A. Reilly, R. Bellisario, and K. A. Pass
Multivariate discrimination for phenylketonuria (PKU) and non-PKU hyperphenylalaninemia after analysis of newborns' dried blood-spot specimens for six amino acids by ion-exchange chromatography
Clin. Chem., February 1, 1998; 44(2): 317 - 326.
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Copyright © 1984 by the American Association for Clinical Chemistry.