Clinical Chemistry
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Clinical Chemistry 30: 311-314, 1984;
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Clinical Chemistry, Vol 30, 311-314, Copyright © 1984 by American Association for Clinical Chemistry

Effect of contrast media on radioimmunoassay of beta-endorphin in cerebrospinal fluid

VS Fang, RG Fessler, JR Rachlin and FD Brown

An effect of metrizamide, a contrast medium, on results of beta- endorphin radioimmunoassay was examined. We found that 1, 5, and 10 microL of the medium added to 100 microL of standard containing 0 to 500 pg of beta-endorphin shifted the standard curve to the left in proportion to the metrizamide concentration. Three other contrast media showed a similar effect at low concentrations of beta-endorphin. This effect of contrast media artificially increased results in radioimmunoassay of beta-endorphin in cerebrospinal fluid, the mean overestimate being 121.9% (range, 0 to 435%). For plasma samples, this effect of contrast media resulted in an average 11.7% overestimate of beta-endorphin (range, -16% to 41%). These observations bring into question the validity of a previous suggestion that an increase in beta- endorphin in cerebrospinal fluid after intracerebral electrical stimulation is the mechanism for stimulation-produced analgesia.


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R. Fessler, F. Brown, Rachlin JR, S Mullan, and V. Fang
Elevated beta-endorphin in cerebrospinal fluid after electrical brain stimulation: artifact of contrast infusion?
Science, June 1, 1984; 224(4652): 1017 - 1019.
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Copyright © 1984 by the American Association for Clinical Chemistry.