Clinical Chemistry
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Clinical Chemistry 30: 316-318, 1984;
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Clinical Chemistry, Vol 30, 316-318, Copyright © 1984 by American Association for Clinical Chemistry

Sources of error in determinations of carnitine and acylcarnitine in plasma

RC Fishlock, LL Bieber and AM Snoswell

Radioactive and nonradioactive L-carnitine and acyl-L-carnitine were used to evaluate the washing procedures used during the determination of free, total, short-chain, and long-chain acylcarnitine in human and sheep plasma. The volume of fluid trapped by the protein precipitated by perchloric acid is approximately 24% of the total fluid volume and thus contains 24% of free carnitine and short-chain acylcarnitine. Washing twice with distilled water removes about 25% of the long-chain acylcarnitine along with the trapped free carnitine and short-chain acylcarnitines. Washing the pellet twice with a 60 g/L solution of perchloric acid completely removes the trapped free carnitine and short- chain acylcarnitine but does not remove the bound long-chain acylcarnitines. Thus washing with perchloric acid is essential for accurate measurement of long-chain acylcarnitines in plasma samples.


The following articles in journals at HighWire Press have cited this article:


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 CirculationHome page
G. Brevetti, F. di Lisa, S. Perna, R. Menabo, R. Barbato, V. Domenico Martone, and N. Siliprandi
Carnitine-Related Alterations in Patients With Intermittent Claudication : Indication for a Focused Carnitine Therapy
Circulation, May 1, 1996; 93(9): 1685 - 1689.
[Abstract] [Full Text]


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Copyright © 1984 by the American Association for Clinical Chemistry.