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Clinical Chemistry 30: 349-351, 1984;
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Clinical Chemistry, Vol 30, 349-351, Copyright © 1984 by American Association for Clinical Chemistry

Rapid assessment of the distribution of apolipoproteins C-II and C-III subspecies in triglyceride-rich lipoproteins by isoelectric focusing

MJ Bugugnani, M Koffigan, I Kora, D Ouvry, V Clavey and JC Fruchart

We describe an isoelectric-focusing method for rapidly separating and quantifying apolipoprotein (apo) C-II and C-III subspecies of triglyceride-rich lipoproteins. Concentrated very-low-density lipoproteins (VLDL) or delipidated apo VLDL are focused on dry acrylamide plates, after their rehydration with ampholytes and urea. Apo C-II and apo C-III in VLDL are resolved into four major bands--C-II (pI 5.01), C-III0 (pI 5.10), C-III1 (pl 4.92), and C-III2 (pI 4.84)--at the same pI values as for purified apo C. This precise technique can be used without delipidating VLDL. The relative percentage of C apoproteins found in VLDL from plasma of normal subjects agreed with previously published data. The ratio of apo C-II to apo C-III decreased in patients with chronic renal failure or with coronary artery disease.


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 J. Lipid Res.Home page
J.-F. Mauger, P. Couture, N. Bergeron, and B. Lamarche
Apolipoprotein C-III isoforms: kinetics and relative implication in lipid metabolism
J. Lipid Res., June 1, 2006; 47(6): 1212 - 1218.
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Copyright © 1984 by the American Association for Clinical Chemistry.