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Clinical Chemistry 30: 387-390, 1984;
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Clinical Chemistry, Vol 30, 387-390, Copyright © 1984 by American Association for Clinical Chemistry

Development of an agarose gel electrophoresis technique for determining alpha-amylase isoenzymes

VL Royse and DM Jensen

Evaluation of alpha-amylase isoenzymes as a clinical diagnostic aid by previous methodologies has been either too insensitive or too cumbersome for routine clinical laboratory use. With use of an agarose gel electrophoresis system (Worthington Diagnostic Systems, Inc.) serum amylase activity can easily be resolved into at least nine isoenzymes, the resolution being comparable with that of isoelectric focusing. Samples can be analyzed in less than one working day, with use of conventional reagents.


The following articles in journals at HighWire Press have cited this article:


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R.B. Field, A.I. Spielman, and A.R. Hand
Purification of Lingual Amylase from Serous Glands of Rat Tongue and Characterization of Rat Lingual Amylase and Lingual Lipase
Journal of Dental Research, February 1, 1989; 68(2): 139 - 145.
[Abstract] [PDF]


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Copyright © 1984 by the American Association for Clinical Chemistry.